100 mM dUTP

Thermo Scientific dUTP (2′-deoxyuridine 5′-triphosphate) is a very stable nucleotide, supplied as a 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH. The nucleotide is over 99% pure, free from nuclease activities, human DNA and E. coli. It is designed for many different molecular biology applications.

Reflexes

• Purity greater than 99%, confirmed by HPLC
• Free of human DNA and E. coli
• Highly stable

Application tested in:

• Long-range PCR (40 kb)
• Synthesis of cDNA and RT-PCR
• Real-time PCR
• standard PCR
• high fidelity PCR
• Applications
• For use in all molecular biology applications, including PCR, real-time PCR, and RT-PCR with Taq DNA polymerase, cDNA synthesis, and primer extension.

Note:

dUTP is not suitable for PCR with proofreading enzymes or enzyme mixtures containing proofreading enzymes.
For research use only. It should not be used in diagnostic procedures.

Specifications

  • For use with (application): PCR amplification
  • Label or Dye: Unconjugated
  • Concentration: 100 mM
  • Label type: No tag

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