100 mM dUTP
Thermo Scientific dUTP (2′-deoxyuridine 5′-triphosphate) is a very stable nucleotide, supplied as a 100 mM aqueous solution titrated to pH 7.3-7.5 with NaOH. The nucleotide is over 99% pure, free from nuclease activities, human DNA and E. coli. It is designed for many different molecular biology applications.
Reflexes
• Purity greater than 99%, confirmed by HPLC
• Free of human DNA and E. coli
• Highly stable
Application tested in:
• Long-range PCR (40 kb)
• Synthesis of cDNA and RT-PCR
• Real-time PCR
• standard PCR
• high fidelity PCR
• Applications
• For use in all molecular biology applications, including PCR, real-time PCR, and RT-PCR with Taq DNA polymerase, cDNA synthesis, and primer extension.
Note:
dUTP is not suitable for PCR with proofreading enzymes or enzyme mixtures containing proofreading enzymes.
For research use only. It should not be used in diagnostic procedures.
Specifications
- For use with (application): PCR amplification
- Label or Dye: Unconjugated
- Concentration: 100 mM
- Label type: No tag
