Histo-Clear II, like its predecessor Histo-Clear
Histo-Clear II, like its predecessor Histo-Clear, is a safer histological clearing agent that leads to the manufacturing of high-quality tissue slides. Histo-Clear II is unhazardous and totally biodegradable, thus decreasing disposal costs. Histo-Clear II has a vastly diminished citrus odor as compared with totally different citrus-based histological clearing brokers.
Histo-Clear II
- Low Hazard
- Biodegradable
- Lowered Citrus Odor
Primarily probably the most usually used histological stain, hematoxylin is a pure compound extracted from a species of tree current in Mexico and the West Indies. The extracted compound is oxidized to supply hematein, the energetic staining ingredient of the hematoxylin stain.
The manufacturing of histological staining choices is an art work. Just some laboratories are in a position to making a stain of reliable vibrance. At Nationwide Diagnostics we’ve now gone one step further. Our proprietary methods acquire the most effective administration of the ripening course of, resulting in the one commercially on the market Harris’ Hematoxylin with a full one 12 months shelf life.
Discover: Histo-Clear II is simply not appropriate with many synthetic mounting media. Histo-Clear II is a long-standing favorite dry mounting procedures and aqueous mounting. Please affirm compatibility alongside along with your mounting media sooner than using Histo-Clear II with in wet-mounting with synthetic media.
Hazardous Provides Cost (HazMat) applies if shipped by Air.
PROCEDURE
Discover: The following protocol has given good to fantastic outcomes with all types of tissue sections. It
may ought to be optimized for a given tissue, half
thickness, and so forth.
1. Dewax and rehydrate slides
a) Immerse slides inside the following sequence of baths:
l Histo-Clear – 10 minutes
l Histo-Clear – 10 minutes
l 100% ethanol – 2 minutes
l 95% ethanol – 2 minutes
l 50% ethanol – 2 minutes
l Water – 2 minutes
2. Stain
a) Immerse slides in Hematoxylin reply for 10- 20 minutes.
b) Rinse 2 minutes in water or Scott’s Tapwater substitute.
c) Differentiate with 2-5 dips in 70% ethanol containing 0.1% HCl.
d) Immerse for 2 minutes in Scott’s Tapwater substitute.
e) Stain in 1% aqueous Eosin with 0.1 acetic acid for 10 minutes.
3. Dehydrate, clear and mount
a) Immerse slides inside the following sequence of
baths:
l 50% ethanol – 2 minutes
l 95% ethanol – 2 minutes
l 100% ethanol – 2 minutes
l Histo-Clear – 10 minutes
l Histo-Clear – 10 minutes
b) Mount coverslip with Histomount.
Histo-Clear II |
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| NAT1334 | National Diagnostics | 1 US gallon | EUR 126 |
Histo-Clear II |
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| NAT1336 | National Diagnostics | 5 US gallons | EUR 381 |
Calci-Clear |
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| NAT1312 | National Diagnostics | EACH | EUR 78 |
Calci-Clear |
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| NAT1314 | National Diagnostics | EACH | EUR 128 |
Calci-Clear |
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| NAT1316 | National Diagnostics | EACH | EUR 386 |
Calci-Clear Rapid |
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| NAT1318 | National Diagnostics | EACH | EUR 75 |
Calci-Clear Rapid |
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| NAT1320 | National Diagnostics | EACH | EUR 128 |
Calci-Clear Rapid |
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| NAT1322 | National Diagnostics | EACH | EUR 386 |
10X PBS MaxTag Histo (OKRA00043) |
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| OKRA00043 | Aviva Systems Biology | 100 ml | EUR 140 |
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Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
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Hematoxylin Counterstain MaxTag Histo (OKRA00050) |
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| OKRA00050 | Aviva Systems Biology | 15 ml | EUR 140 |
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Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
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Diogenes Kit |
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| NAT1000 | National Diagnostics | 1KIT | EUR 225 |
Hydrogen Peroxide Assay Kit |
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| NAT1002 | National Diagnostics | 1KIT | EUR 257 |
ProtoBlock System |
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| NAT1004 | National Diagnostics | 1KIT | EUR 102 |
450ML PBS 10X |
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| NAT1006 | National Diagnostics | 450ML | EUR 80 |
4L PBS 10X |
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| NAT1008 | National Diagnostics | 4L | EUR 199 |
ProtoGlow ECL 200ml Kit |
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| NAT1010 | National Diagnostics | EACH | EUR 295 |
ProtoGlow ECL 500ml Kit |
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| NAT1012 | National Diagnostics | EACH | EUR 523 |
100G Acrylamide |
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| NAT1014 | National Diagnostics | 100G | EUR 100 |
500G Acrylamide |
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| NAT1016 | National Diagnostics | 500G | EUR 218 |
1KG Acrylamide |
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| NAT1018 | National Diagnostics | 1KG | EUR 344 |
25G AquaPor LE |
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| NAT1020 | National Diagnostics | 25G | EUR 110 |
100G AquaPor LE GTAC |
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| NAT1022 | National Diagnostics | 100G | EUR 209 |
500G AquaPor LE GTAC |
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| NAT1024 | National Diagnostics | 500G | EUR 732 |
25G AquaPor ES |
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| NAT1026 | National Diagnostics | 25G | EUR 127 |
100G AquaPor ES |
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| NAT1028 | National Diagnostics | 100G | EUR 292 |
25G AquaPor LM |
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| NAT1030 | National Diagnostics | 25G | EUR 240 |
100G AquaPor LM |
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| NAT1032 | National Diagnostics | 100G | EUR 717 |
