Histo-Clear II, like its predecessor Histo-Clear
Histo-Clear II, like its predecessor Histo-Clear, is a safer histological clearing agent that leads to the manufacturing of high-quality tissue slides. Histo-Clear II is unhazardous and totally biodegradable, thus decreasing disposal costs. Histo-Clear II has a vastly diminished citrus odor as compared with totally different citrus-based histological clearing brokers.
Histo-Clear II
- Low Hazard
- Biodegradable
- Lowered Citrus Odor
Primarily probably the most usually used histological stain, hematoxylin is a pure compound extracted from a species of tree current in Mexico and the West Indies. The extracted compound is oxidized to supply hematein, the energetic staining ingredient of the hematoxylin stain.
The manufacturing of histological staining choices is an art work. Just some laboratories are in a position to making a stain of reliable vibrance. At Nationwide Diagnostics we’ve now gone one step further. Our proprietary methods acquire the most effective administration of the ripening course of, resulting in the one commercially on the market Harris’ Hematoxylin with a full one 12 months shelf life.
Discover: Histo-Clear II is simply not appropriate with many synthetic mounting media. Histo-Clear II is a long-standing favorite dry mounting procedures and aqueous mounting. Please affirm compatibility alongside along with your mounting media sooner than using Histo-Clear II with in wet-mounting with synthetic media.
Hazardous Provides Cost (HazMat) applies if shipped by Air.
PROCEDURE
Discover: The following protocol has given good to fantastic outcomes with all types of tissue sections. It
may ought to be optimized for a given tissue, half
thickness, and so forth.
1. Dewax and rehydrate slides
a) Immerse slides inside the following sequence of baths:
l Histo-Clear – 10 minutes
l Histo-Clear – 10 minutes
l 100% ethanol – 2 minutes
l 95% ethanol – 2 minutes
l 50% ethanol – 2 minutes
l Water – 2 minutes
2. Stain
a) Immerse slides in Hematoxylin reply for 10- 20 minutes.
b) Rinse 2 minutes in water or Scott’s Tapwater substitute.
c) Differentiate with 2-5 dips in 70% ethanol containing 0.1% HCl.
d) Immerse for 2 minutes in Scott’s Tapwater substitute.
e) Stain in 1% aqueous Eosin with 0.1 acetic acid for 10 minutes.
3. Dehydrate, clear and mount
a) Immerse slides inside the following sequence of
baths:
l 50% ethanol – 2 minutes
l 95% ethanol – 2 minutes
l 100% ethanol – 2 minutes
l Histo-Clear – 10 minutes
l Histo-Clear – 10 minutes
b) Mount coverslip with Histomount.
Histo-Clear II |
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| NAT1334 | Scientific Laboratory Supplies | EACH | EUR 111.72 |
Histo-Clear II |
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| NAT1336 | Scientific Laboratory Supplies | EACH | EUR 466.26 |
10X PBS MaxTag Histo (OKRA00043) |
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| OKRA00043 | Aviva Systems Biology | 100 ml | EUR 168 |
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Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
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Hematoxylin Counterstain MaxTag Histo (OKRA00050) |
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| OKRA00050 | Aviva Systems Biology | 15 ml | EUR 168 |
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Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
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25ML Formamide CE |
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| NAT1096 | National Diagnostics | 25ML | Ask for price |
4L ScintLogic U |
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| NAT1532 | National Diagnostics | 4L | EUR 220.8 |
20L FlowLogic U |
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| NAT1534 | National Diagnostics | 20L | EUR 907.2 |
Test product |
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| testtt | National Diagnostics | 0 | Ask for price |
Streptavidin-Peroxidase Conjugate MaxTag Histo (OKRA00036) |
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| OKRA00036 | Aviva Systems Biology | 1 each | EUR 339.6 |
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Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
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PolyMount Mounting Media MaxTag Histo (OKRA00045) |
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| OKRA00045 | Aviva Systems Biology | 15 ml | EUR 151.2 |
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Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
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384 Clear Wells Clear Frame |
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| PCR0430 | Scientific Laboratory Supplies | PK50 | EUR 248.52 |
Biotin anti-IgG Antibody MaxTag Histo (OKRA00041) |
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| OKRA00041 | Aviva Systems Biology | 1 each | EUR 339.6 |
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Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
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Calci-Clear |
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| NAT1312 | Scientific Laboratory Supplies | EACH | EUR 38.08 |
Calci-Clear |
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| NAT1314 | Scientific Laboratory Supplies | EACH | EUR 107.16 |
Calci-Clear |
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| NAT1316 | Scientific Laboratory Supplies | EACH | EUR 451.44 |
Centrifuge Tube |
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| C1005-T5*-Clear | Benchmark Scientific | 1 PC | EUR 117.16 |
Histo-Blood Group ABO System Transferase (ABO) Antibody |
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| 20-abx141615 | Abbexa |
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Histo-Blood Group ABO System Transferase (ABO) Antibody |
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| 20-abx001336 | Abbexa |
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Histo-Blood Group ABO System Transferase (ABO) Antibody |
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| abx117115-100ug | Abbexa | 100 ug | EUR 560.4 |
Histo-Blood Group ABO System Transferase (ABO) Antibody |
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| abx029931-400ul | Abbexa | 400 ul | EUR 627.6 |
Histo-Blood Group ABO System Transferase (ABO) Antibody |
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| abx029931-80l | Abbexa | 80 µl | EUR 343.2 |
Histo-Blood Group ABO System Transferase (ABO) Antibody |
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| 20-abx315565 | Abbexa |
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Histo-Blood Group ABO System Transferase (ABO) Antibody |
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| 20-abx339880 | Abbexa |
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Histo-Blood Group ABO System Transferase (ABO) Antibody |
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| 20-abx339881 | Abbexa |
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Histo-Blood Group ABO System Transferase (ABO) Antibody |
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| abx215750-100ug | Abbexa | 100 ug | EUR 526.8 |
Biotin anti-Rabbit IgG Antibody MaxTag Histo (OKRA00042) |
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| OKRA00042 | Aviva Systems Biology | 1 each | EUR 339.6 |
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Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
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Biotin anti-Human IgG Antibody MaxTag Histo (OKRA00047) |
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| OKRA00047 | Aviva Systems Biology | 1 each | EUR 339.6 |
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Description: Description of target: ;Species reactivity: ;Application: ;Assay info: ;Sensitivity: |
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