Swine Rapid

Summary

African swine fever virus (ASFV) is the pathogen of African swine rapid fever, a deadly and highly contagious disease that affects wild boars and domestic pigs. The flow of ASFV through pork products is more hidden, riskier, and more difficult to prevent and control. At present, methods for in situ detection of ASFV in preclinically infected pigs and circulating porcine products are lacking.

Here, a fluorescent dipstick-based rapid detection method for ASFV in pork combined with recombinase-assisted amplification (RAA) and quantum dot microspheres (QDM) was established. This method is specific for ASFV without cross-reactivity with pseudorabies virus (PRV), porcine circovirus type 2 (PCV2), and porcine reproductive and respiratory syndrome virus (PRRSV). The method also showed high sensitivity with a detection limit of 1 copy for ASFV plasmid templates containing the B646L gene and 100 copies/g for DNA extracts from pig clinical samples in a short detection time of fewer than 25 minutes.

In addition, the method showed 99.17% consistency with real-time PCR in the detection of ASFV from 120 pig clinical samples. In general, the QDM-based dipstick method provides specific, sensitive, rapid, and easy detection of ASFV in pork, which can help maintain the food safety of pork products and facilitate inspection. traceability and prevention of African swine fever virus. Rapid and sensitive detection of African swine fever virus in pork using a QDM-based dipstick assay.

Keywords:

In situ detection of African swine fever virus; Pork samples; Quantum Dot Microsphere (QDM) based test strip; Recombinase-assisted amplification (RAA).

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