AffiVET® Poultry Avian Influenza Virus AIV Antigen Rapid Tests

Test Principal:

The AffiVET® Rapid AIV Ag Test Kit utilizes chromatographic immunoassay technology to qualitatively detect avian influenza type A virus antigen in chicken or duck (excluding wild animal) cloaca, oropharynx, or feces sample.

The device features a test (T) line and a control (C) line, where the control line serves as a reference ensuring the test is functioning correctly.

The appearance of the control line confirms proper test execution.

Highly selective antibodies specific to avian influenza type A virus are employed in both capture and detection roles within the assay, enabling accurate detection of AIV antigen in chicken or duck (excluding wild animal) sample with high accuracy.

Material Required:

Storage & Stability:

• Store the test kit at 2~30°C. DO NOT FREEZE.
• Do not store the test kit in the direct
  sunlight.
• The test kit is stable within the expiration date marked on the package label.
  

Sample collection & Preparation:

1. Use samples from chicken or duck (excluding wild animal) cloaca, oropharynx, or feces sample to ensure accurate detection of AIV antigen.
2. If the sample cannot be tested immediately, refrigerate it at 2-8°C for up to 24 hours. For longer storage, freeze the sample at -20°C or below. Frozen samples should be brought to room temperature (15~30°C) prior to use.

Test Procedure:

1. Bring all kit components and samples to room temperature before use to ensure optimal performance.
   
2. Collect samples using a swab for accurate testing.
   
3. Insert the swab into the assay diluent tube and gently mix it for 10 seconds.
   
4. Wait for 1 minute to allow the samples to settle down before proceeding with the test.
   
5. Remove the test device from the foil pouch and place it on a flat, dry surface.
   
6. Using the disposable dropper provided, extract the supernatant sample from the tube.
   
7. Apply (4) four drops of the sample into the sample hole on the test device, adding each drop one at a time.
   
8. Start the timer and observe as the sample flows across the result window. If the sample does not appear after 1 minute, add one more drop of the prepared sample to the sample hole.
9. Interpret test results at 20 minutes. Do not read the results after 30 minutes.
   

Result Interpretation:

Negative result

This means that there is no AIV antigens in the sample or the level is lower than the test detection limit.

Positive result:

This confirms the presence of the AIV antigens in the sample.

Invalid Result:

Absence of C-Line
Presence of T-Line

Or

Absence of C-Line
Absence of T-Line

This means that the procedure was not applied correctly or that the test is invalid.

Cautions:

• The AffiVET® Rapid AIV Ag Test Kit is intended for use with chickens or ducks (excluding wild animals) only. Do not use for other animals.
  
• The test device is sensitive to both humidity and heat. Perform the test immediately after removing the test device from the foil pouch.
  
• Do not reuse any test components.
  
• Apply the assay diluent vertically.
  
• Avoid touching the membrane within the result window of the test device.
  
• Do not use the test kit beyond the stated expiration date marked on the package label.
  
• Do not use the test kit if the pouch is damaged or if the seal is broken.
  
• Do not mix components from different lot numbers. The components in this kit have been quality control tested as a standard batch unit.
  
• Handle all samples as potentially infectious. Wear protective gloves when handling samples and wash hands thoroughly afterward.
  
• Decontaminate and dispose of all samples, used kits, and potentially contaminated materials safely in accordance with national and local regulations.